The purpose of this study was to investigate the morphological characters of male and female Acrossocheilius hemispinus.Principal component analysis, R-cluster analysis, Stepwise discriminant analysis and T-test were used to analyze 17 measured characters and 14 standardized character traits of 60 individuals.The result of principal component analysis showed that the contribution rates of the three extracted principal components were 70.056%, 8.284% and 4.674%, respectively, while the cumulative contribution rates reached 83.014%.4 characters were screened out from 14 standardized morphological characters of 60 A.hemispinus by stepwise discriminant analysis to establish the sex discriminant equation (Male: F₁=917.211X₁+78.772X₂+1 898.439X₃+814.049X₄-621.398, Female: F₂=976.079X₁+71.879X₂+1 809.187X₃+774.359X₄-610.235).The comprehensive discriminant accuracy rate for male and female population was 81.7%.The T-test showed that the trunk length/body length, caudal peduncle length/body length, caudal peduncle depth/body length and head depth/head length were significantly different between male and female populations (P<0.01).Female had longer trunk and tail, and male had higher caudal peduncle and head.